- the same restriction enzyme is used to cut out insulin gene from human DNA and plasmid.
- the insulin gene is joined together with the plasmid by ligase enzyme.
- recombinant DNA is formed and inserted into a bacterial cell, which acts as the vector.
- transgenic bacteria are complete and grown in a fermenter to reproduce and duplicate.
*transgenic: if something is transgenic it means they contain genes transferred from another species.
Uses of Manufactured Insulin:
- helps those with diabetes as their body does not produce enough insulin
- the insulin can be injected to control blood glucose levels by converting glucose to glycogen, which is insoluble, and can be stored in muscles/liver
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