IGCSE Chemistry

Wednesday, March 18, 2020

5.14 understand how large amounts of human insulin can be manufactured from genetically modified bacteria that are grown in a fermenter

Manufacturing of Insulin:
- the same restriction enzyme is used to cut out insulin gene from human DNA and plasmid.
- the insulin gene is joined together with the plasmid by ligase enzyme. 
- recombinant DNA is formed and inserted into a bacterial cell, which acts as the vector.
- transgenic bacteria are complete and grown in a fermenter to reproduce and duplicate.

*transgenic: if something is transgenic it means they contain genes transferred from another species.

Uses of Manufactured Insulin:
- helps those with diabetes as their body does not produce enough insulin
- the insulin can be injected to control blood glucose levels by converting glucose to glycogen, which is insoluble, and can be stored in muscles/liver

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